CARIPARO Excellence Project 2024-2026
Exploring bone marrow in T-LGL leukemia: the hive of activity addressing disease onset, progression and concurrent dysplasia unbalances
- PI Prof. Renato Zambello (DIMED, UniPD)
- Dr. Antonella Teramo
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Dr. Giulia Calabretto
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Dr. Elisa Rampazzo
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Dr. Elena Buson
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Dr. Valentina Trimarco
- Prof. Stefania Bortoluzzi (DISCOG, UniPD)
- Dr. Enrico Gaffo
- Dr. Ilias Glogovitis
- Dr. Silvia Orsi
- Prof. Marco Pizzi (DIMED, UniPD)
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Dr. Vincenza Guzzardo
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Dr. Federico Scarmozzino
Short project description
T-LGLL (large granular lymphocyte leukemia) is a chronic lymphoproliferative disorder presenting with cytopenias that are linked to impaired immune system and related morbidity. Recent studies showed bone marrow dysplastic alterations, in some T-LGLL cases, with a risk of progression to myelodysplastic syndromes (MDS). T-LGLL originates in the bone marrow (BM), where LGLs encounter potential triggering antigens, and where the processes leading to cytopenias and dysplastic alterations take place.
Our project aims to characterize the BM niche in T-LGLL compared to healthy controls, combining single-cell RNA sequencing (RNA-seq) on hematopoietic BM cells and spatial transcriptomic analysis plus multiplexed immunohistochemistry on BM biopsies.
We are using cutting-edge technologies, in combination with bioinformatic and artificial intelligence approaches, to portray the features of the BM in unprecedented detail, ultimately translating into new therapeutic targets for T-LGLL and related disorders.